Cell Viability Assay试剂盒(Fluorometric - Near InfraRed) (ab112123)

概述

  • 产品名称Cell Viability Assay试剂盒(Fluorometric - Near InfraRed)
    参阅全部 Cell Viability 试剂盒
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Direct
  • 产品概述

    Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. The proprietary far red fluorescent dye used in the kit is a hydrophobic compound that can easily permeate intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly red fluorescent hydrophilic product that is well-retained in the cell cytoplasm. When well excited with the He-Ne Laser at 633 nm, the red fluorophore emits intense red fluorescence at ~660 nm.

    ab112123 is an effective tool of labeling cells for the investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112123 is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. It comes with reagents sufficient to perform 200 assays in a 96-well plate or 800 assays in a 384-well plate. The kit provides all the essential components with an optimized protocol for microplate reader analysis of cell viability.

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  • 说明

    ab112123 should be stored desiccated.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.

应用

Our Abpromise guarantee covers the use of ab112123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

Cell Viability Assay Kit (Fluorometric - Near InfraRed) 图像

  • CHO-K1 cell number response measured with ab112123.
    CHO-K1 cells at 0 to 50,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Near InfraRed Dye solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 635/670 nm.

实验方案

Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123)参考文献

ab112123 has not yet been referenced specifically in any publications.

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Le filtre dufluorophore correspondant du kit ab112123 est le filtre pourCy5. Lesfiltres pour leCy3 et le Texas Red ne sont donc pas appropriés pour l'utilisation du kit ab112123.

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