Cell Viability Assay试剂盒(Bioluminescent) (ab65314)

概述

  • 产品名称Cell Viability Assay试剂盒(Bioluminescent)
    参阅全部 Cell Viability 试剂盒
  • 测试
    200 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • 检测类型Direct
  • 灵敏度
    > 10 cells/well
  • 范围
    10 cells/well - 100 cells/well
  • 检测时间
    0h 30m
  • 产品概述

    Abcam's Cell Viability Assay Kit (Bioluminescent) utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in cell has been used as an indicator of cell death. In contrast, cell proliferation has been recognized by increased levels of ATP.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.

应用

Our Abpromise guarantee covers the use of ab65314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Cell Viability Assay Kit (Bioluminescent) (ab65314)参考文献

ab65314 has not yet been referenced specifically in any publications.

Product Wall



I've confirmed with the lab, and you can use this kind of media but be sure to use a control media with the same amount of RPMI and FBS to account for any signals generated due to these 2 in your sample readings. Just subtract the control rea...

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Hello!

You can use a control media with the same amount of RPMI and FBS to account for any signals generated due to these 2 in your sample readings. Just subtract the readings you get in the controls from the standards.

Please let me ...

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Yes, you are correct. Lower ATP readings would indicate a decrease in cell viability.

Ab65314 is recommended for cell viability. For media, use the same protocol as mentioned for the suspension cells in step 2.a.

Thank you for contacting us and for asking your question about the Cell Viability Assay Kit instructions.

The instruction on page 7 of the protocol to read the samples for one minute after adding the enzyme is mistaken. It should read:
...

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Thank you for your reply. I can confirm that conventional scintillation counters can not be used for measurements with this kit. However, when Beta Counter is used it should be programmed in the “out of coincidence” (or Luminescence mode) for m...

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Thank you for your enquiry. I can confirm that the read out of ab65314  Cell Viability Assay Kit is via luminescence. Therefore, we would suggest to use the indicated equipment which is a luminometer.  Theoretically a luminometer and a scintillation ...

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Thank you for your enquiry. I have contacted the originator of this kit who have confirmed that it can be used for measuring the ATP in mitochondrial lysates. Once you have produced your mitoochondria lysates, you can follow the kit protocol and ge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"