Cell Cytotoxicity Assay试剂盒(Fluorometric) (ab112119)

概述

  • 产品名称Cell Cytotoxicity Assay试剂盒(Fluorometric)
    参阅全部 Cell Cytotoxicity 试剂盒
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 种属反应性
    与反应: Human, Chinese Hamster
    预测可用于: all Mammals
  • 产品概述

    The measurement of mitochondrial dehydrogenases (e.g. LDH) activity is a well-accepted assay to quantify cell numbers and monitor cell viability.
    ab112119 Cell Cytotoxicity Assay Kit provides a fast, simple, accurate and homogeneous assay for the fluorimetric detection of viable cells. ab112119 is based on the observation that oxidized non-fluorescent blue resazurin is reduced to a red fluorescent dye (resorufin) by accepting an electron from mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells.

    ab112119 is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are quite stable with minimal cytotoxicity, thus a longer incubation time (such as 24-48 hours) is possible if required.
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  • 说明

    ab112119 should be stored desiccated

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab112119 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Cell Cytotoxicity Assay Kit (Fluorometric) 图像

    • CHO-K1 cell number response was measured with ab112119. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 °C. The absorbance intensity was measured at 570 nm and 605 nm. The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit from the blank controls was 60 cells/well (n=6).

    实验方案

    Cell Cytotoxicity Assay Kit (Fluorometric) (ab112119)参考文献

    ab112119 has not yet been referenced specifically in any publications.

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