Cell Cytotoxicity Assay试剂盒(Colorimetric) (ab112118)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Cell Cytotoxicity Assay试剂盒(Colorimetric)
参阅全部 Cell Cytotoxicity 试剂盒 -
检测方法
Colorimetric -
样品类型
Adherent cells, Suspension cells -
检测类型
Quantitative -
种属反应性
与反应: Mammals, Other species -
产品概述
Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions. There are a variety of parameters that can be used. ab112118 uses a proprietary water-soluble dye that changes its absorption spectra upon cellular reduction. The absorption ratio change is directly proportional to the number of living cells. The characteristics of its high sensitivity, non-radioactivity and no-wash method make ab112118 suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.
ab112118 does not require pre-mixing of components and has higher sensitivity compared to tetrazolium based colorimetric assays (such as MTT and XTT). It comes with reagents sufficient to run 1000 assays. The kit components are quite stable with minimal cytotoxicity, thus longer incubation times (such as 24-48 hours) are possible if required.
ab112118 is robust and convenient to use. It can be readily adapted for a wide variety of instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Visit our FAQs page for tips and troubleshooting.
Cytotoxicity assay protocol summary:
- add assay solution to cells and shake for 30 s
- incubate for 1-24 hrs
- analyze with microplate reader -
说明
ab112118 should be stored desiccated.
As low as 300 cells can be accurately quantified using ab112118.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1000 tests Assay Solution Component A 1 x 20ml -
研究领域
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图片
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•HeLa: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.
•NIH3T3: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.
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CHO-K1 cell number response was measured with ab112118. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 oC. The absorbance intensity was measured at 570 nm and 605 nm. The ratio of OD570/OD605 is proportional to the number of cells as indicated.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab112118 被引用在 23 文献中.
- Khan A et al. Safety, Stability, and Therapeutic Efficacy of Long-Circulating TQ-Incorporated Liposomes: Implication in the Treatment of Lung Cancer. Pharmaceutics 14:N/A (2022). PubMed: 35057049
- Mateo F et al. Modification of BRCA1-associated breast cancer risk by HMMR overexpression. Nat Commun 13:1895 (2022). PubMed: 35393420
- Ma L et al. Effect of glycerol addition time on the cryopreserved Korean native brindle cattle (Chikso) sperm quality. Anim Reprod 19:e20210058 (2022). PubMed: 35432606
- Matveeva VG et al. Advantages of Fibrin Polymerization Method without the Use of Exogenous Thrombin for Vascular Tissue Engineering Applications. Biomedicines 10:N/A (2022). PubMed: 35453539
- Lajqi T et al. Gram-positive Staphylococcus aureus LTA promotes distinct memory-like effects in murine bone marrow neutrophils. Cell Immunol 376:104535 (2022). PubMed: 35537323