Cell Cycle Assay试剂盒(Fluorometric - Green) (ab112116)

概述

  • 产品名称
    Cell Cycle Assay试剂盒(Fluorometric - Green)
    参阅全部 Cell Cycle 试剂盒
  • 检测方法
    Fluorescent
  • 样品类型
    Adherent cells, Suspension cells
  • 实验步骤
    One step assay
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Mammal
  • 产品概述

    Cell Cycle Assay Kit (Fluorometric - Green) (ab112116) is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Green CCS1 can be monitored with a flow cytometer at Ex/Em = 490 nm/520 nm (FL1 channel).

    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用
    适用于: Functional Studies, Flow Cytmore details
  • 平台
    Flow

性能

应用

Our Abpromise guarantee covers the use of ab112116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

图片

  • Histogram of HeLa cells (black: untreated cells, red : apoptotic cells) stained with 0.1x Nuclear Green CCS1

  • DNA profile in growing and camptothecin treated Jurkat cells.

    Jurkat cells were treated without (red) or with 20 µM camptothecin (blue) in a 37°C, 5% CO2 incubator for ~8 hours, then dye loaded with 200X Green Fluorescence Probe for 60 minutes. The fluorescence intensity of 200X Green Fluorescence Probe was measured with a flow cytometer using the FL1 channel.

实验方案

文献

This product has been referenced in:
  • Sun X & Zhang L MicroRNA-143 suppresses oral squamous cell carcinoma cell growth, invasion and glucose metabolism through targeting hexokinase 2. Biosci Rep 37:N/A (2017). Human . Read more (PubMed: 28174335) »
  • García-Castro B  et al. Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines. BMC Cancer 13:557 (2013). Human . Read more (PubMed: 24274766) »

See all 2 Publications for this product

客户评价及客户问答

Yes, you are right. The two distinct peaks are easy to see G1 and G2, the S phase is between G1 and G2 and it is not peak but more like a flat population, it is much easier to see it if you plot it as linear instead of log on X axis. See the following ...

Read More

Yes, these two kits are not species specific.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册