Cell Cycle Assay试剂盒(Fluorometric - Green) (ab112116)

概述

  • 产品名称Cell Cycle Assay试剂盒(Fluorometric - Green)
    参阅全部 Cell Cycle 试剂盒
  • 检测方法Fluorescent
  • 样品类型
    Adherent cells, Suspension cells
  • 实验步骤One step assay
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    ab112116 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Green CCS1 can be monitored with a flow cytometer at Ex/Em = 490 nm/520 nm (FL1 channel).

    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用适用于: Functional Studies, Flow Cytmore details
  • 平台Flow

性能

    应用

    Our Abpromise guarantee covers the use of ab112116 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.
    Flow Cyt Use at an assay dependent concentration.

    Cell Cycle Assay Kit (Fluorometric - Green) 图像

    • Histogram of HeLa cells (black: untreated cells, red : apoptotic cells) stained with 0.1x Nuclear Green CCS1

    • DNA profile in growing and camptothecin treated Jurkat cells.

      Jurkat cells were treated without (red) or with 20 µM camptothecin (blue) in a 37°C, 5% CO2 incubator for ~8 hours, then dye loaded with 200X Green Fluorescence Probe for 60 minutes. The fluorescence intensity of 200X Green Fluorescence Probe was measured with a flow cytometer using the FL1 channel.

    实验方案

    Cell Cycle Assay Kit (Fluorometric - Green) (ab112116)参考文献

    This product has been referenced in:
    • García-Castro B  et al. Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines. BMC Cancer 13:557 (2013). Human . Read more (PubMed: 24274766) »

    See 1 Publication for this product

    Product Wall

    Yes, you are right. The two distinct peaks are easy to see G1 and G2, the S phase is between G1 and G2 and it is not peak but more like a flat population, it is much easier to see it if you plot it as linear instead of log on X axis. See the following ...

    Read More

    Yes, these two kits are not species specific.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"