概述
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产品名称Anti-CEE抗体
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描述兔多克隆抗体to CEE
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宿主Rabbit
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经测试应用适用于: WB, IPmore details
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种属反应性与反应: Human
预测可用于: Chimpanzee
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免疫原
Synthetic peptide, corresponding to a region within amino acids 277-327 of Human CEE (NP_057033.2)
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阳性对照
- Whole cell lysate from HeLa and 293T cells
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Related Products
应用
Our Abpromise guarantee covers the use of ab93801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IP |
应用说明
IP: Use at 2 - 5µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.
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序列相似性Belongs to the GET4 family.
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细胞定位Cytoplasm > cytosol.
- Information by UniProt
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数据库链接
- Entrez Gene: 51608 Human
- Omim: 612056 Human
- SwissProt: Q7L5D6 Human
- Unigene: 107387 Human
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别名
- C7orf20 antibody
- CGI 20 antibody
- CGI20 antibody
see all
图片
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Western blot - Anti-CEE antibody (ab93801)All lanes : Anti-CEE antibody (ab93801) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CEE antibody (ab93801)Detection of CEE in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93801 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
文献
ab93801 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"