Customer reviews and FAQs

概述

  • 产品名称
    Anti-CEE抗体
  • 描述
    兔多克隆抗体to CEE
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 277-327 of Human CEE (NP_057033.2)

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 2 - 5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能
      Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.
    • 序列相似性
      Belongs to the GET4 family.
    • 细胞定位
      Cytoplasm > cytosol.
    • Information by UniProt
    • 数据库链接
      • 别名
        • C7orf20 antibody
        • CGI 20 antibody
        • CGI20 antibody
        • Conserved edge expressed protein antibody
        • Conserved edge protein antibody
        • Conserved edge-expressed protein antibody
        • GET 4 antibody
        • GET4 antibody
        • GET4_HUMAN antibody
        • Golgi to ER traffic protein 4 homolog (S. cerevisiae) antibody
        • Golgi to ER traffic protein 4 homolog antibody
        • Transmembrane domain recognition complex 35 kDa subunit antibody
        • Transmembrane domain recognition complex 35kDa antibody
        • TRC35 antibody
        • UPF0363 protein C7orf20 antibody
        see all

      Anti-CEE antibody 图像

      • Western blot - CEE antibody (ab93801)
        Western blot - CEE antibody (ab93801)
        All lanes : Anti-CEE antibody (ab93801) at 0.04 µg/ml

        Lane 1 : HeLa whole cell lysate at 50 µg
        Lane 2 : HeLa whole cell lysate at 15 µg
        Lane 3 : HeLa whole cell lysate at 5 µg
        Lane 4 : 293T whole cell lysate at 50 µg

        Developed using the ECL technique

        Predicted band size : 37 kDa


        Exposure time : 3 minutes
      • Immunoprecipitation - CEE antibody (ab93801)
        Immunoprecipitation - CEE antibody (ab93801)
        Detection of CEE in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93801 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

      Anti-CEE antibody (ab93801)参考文献

      ab93801 has not yet been referenced specifically in any publications.

      发表研究结果有使用 ab93801？请让我们知道，以便我们可以引用本数据表中的参考文章。

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