Anti-CEBP gamma抗体(ab54899)

概述

  • 产品名称Anti-CEBP gamma抗体
    参阅全部 CEBP gamma 一抗
  • 描述
    小鼠单克隆抗体to CEBP gamma
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-151 of Human CEBP gamma

性能

应用

Our Abpromise guarantee covers the use of ab54899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites.
  • 序列相似性Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C/EBP gamma antibody
    • CCAAT/enhancer binding protein (C/EBP) gamma antibody
    • CCAAT/enhancer binding protein gamma antibody
    • CCAAT/enhancer-binding protein gamma antibody
    • CEBPG antibody
    • CEBPG_HUMAN antibody
    • GPE1BP antibody
    • IG/EBP 1 antibody
    • IG/EBP1 antibody
    see all

Anti-CEBP gamma antibody 图像

  • All lanes : Anti-CEBP gamma antibody (ab54899) at 1/1000 dilution

    Lane 1 : Mouse adipocyte lysate - untreated
    Lane 2 : Mouse adipocyte lysate - 5nm rapamycin treatment for 48 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Kun Dong

    See Abreview

  • Western blot against tagged recombinant protein immunogen using ab54899 CEBP gamma antibody at 1ug/ml. Predicted band size of immunogen is 43 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Anti-CEBP gamma antibody (ab54899)参考文献

ab54899 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (12.5%)
Sample Mouse Cell lysate - other (adipocyte)
Specification adipocyte
Treatment 5nm rapamycin for 48 hours
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. KUN DONG

Verified customer

提交于 Jan 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"