The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 22144112
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
Expressed at low levels in the lung, kidney and spleen.
Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain.
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta (phospho T235 + T188) antibody (ab52194)Image from Alam M et al., J Biol Chem. 2013;288(43):30892-903. Fig 3.; doi: 10.1074/jbc.M113.477158.
ab52194 staining CEBP Beta (phospho T335 + T188) in Human MDA-MB-468 breast cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde + 2% sucrose and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (in PBS + 1% BSA) for 30 minutes at 37°C.
From left - DAPI (blue), MUC1-C (green), CERP Beta phospho T335 + T188 (red), Merge, Zoom.
Alam M et al. MUC1-C Oncoprotein Activates ERK->C/EBPß Signaling and Induction of Aldehyde Dehydrogenase 1A1 in Breast Cancer Cells. J Biol Chem288:30892-903 (2013).
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Chen CJ et al. Glutamate released by Japanese encephalitis virus-infected microglia involves TNF-a signaling and contributes to neuronal death. Glia60:487-501 (2012).
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