Anti-CEBP Alpha抗体[15C8] (ab15047)

概述

  • 产品名称Anti-CEBP Alpha抗体[15C8]
    参阅全部 CEBP Alpha 一抗
  • 描述
    小鼠单克隆抗体[15C8] to CEBP Alpha
  • 特异性This antibody may cross react with glutamate--ammonia ligase domain containing 1 protein ((Lengsin) Lens glutamine synthase-like) as it has 8/10 aa identical to the immunogen used to generate ab15047 (this protein is part of the Glutamate synthetase family so its compartmentalization to the cytoplasm) unlike CEDPA which is found in the nucleus.
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Hamster, Cow, Xenopus laevis, Schizosaccharomyces pombe, Zebrafish, Goldfish, Aspergillus nidulans, Neurospora crassa
  • 免疫原

    Synthetic peptide corresponding to Mouse CEBP Alpha aa 1-14.
    Sequence:

    MESADFYEVEPRPP


    (Peptide available as ab39791)

  • 表位Amino terminal region.
  • 阳性对照
    • Transfected hu293T cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab15047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Mouse CEBP Alpha peptide (ab39791).
IP Use at an assay dependent concentration.

靶标

  • 功能C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
  • 序列相似性Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch 5 antibody
    • C/EBP alpha antibody
    • C/ebpalpha antibody
    • CAP4 antibody
    • Caspase 8 precursor antibody
    • CBF-A antibody
    • CCAAT Enhancer Binding Protein alpha antibody
    • CCAAT/enhancer binding protein (C/EBP), alpha antibody
    • CCAAT/enhancer-binding protein alpha antibody
    • CEBP antibody
    • CEBP A antibody
    • CEBP alpha antibody
    • Cebpa antibody
    • CEBPA_HUMAN antibody
    • FADD homologous ICE/CED 3 like protease antibody
    • FADD like ICE antibody
    • FLICE antibody
    • ICE like apoptotic protease 5 antibody
    • ICE8 antibody
    • MACH antibody
    • MCH5 antibody
    • MORT1 associated CED 3 homolog antibody
    see all

Anti-CEBP Alpha antibody [15C8] 图像

  • Anti-CEBP Alpha antibody [15C8] (ab15047) at 2 µg/ml + 293 cell lysates transfected with CEBP alpha.

    Predicted band size : 45 kDa

Anti-CEBP Alpha antibody [15C8] (ab15047)参考文献

This product has been referenced in:
  • Khanna-Gupta A  et al. Up-regulation of Translation Eukaryotic Initiation Factor 4E in Nucleophosmin 1 Haploinsufficient Cells Results in Changes in CCAAT Enhancer-binding Protein a Activity: IMPLICATIONS IN MYELODYSPLASTIC SYNDROME AND ACUTE MYELOID LEUKEMIA. J Biol Chem 287:32728-37 (2012). WB . Read more (PubMed: 22851180) »

See 1 Publication for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Thank you for sending the additional details. You mentioned that the human cells were transfected with a plasmid; can you please tell me the species of the gene that the plasmid contained? Was this also human, or some other species, for instance mouse/...

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Thank you for sending the protocol and image of your blot. I am sorry to see that you are not obtaining a band that corresponds to CEBP Alpha. I am having some difficulty reading the blot image notations. Can you please answer a few questions, to he...

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Many thanks for your feedback, I have added a note on the datasheet following your expert advice and would love to find out if indeed the antibody detected the ligase in your cells. Thank you again for your help, I look forward to hearing from you,

As discussed earlier I enclose the links to the databases that I was looking at to find out if the antibody could be cross reacting with other proteins. The protein Glutamate--ammonia ligase domain containing 1 protein (Lengsin) Lens glutamine synth...

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