The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
cELISA: 1/200 - 1/400.
Flow Cyt: Use 1.2µg for 106 cells.
SDS-PAGE: Use 2µg.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CEACAM19 belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. Like all members of the CEACAM family, the CEACAM19 protein is a single-pass type I membrane protein and consists of a single extracellular N domain.
CEACAM19 expression is ubiquitous with highest expression in prostate, uterus, fetal brain, mammary gland, adrenal gland, skeletal muscle, small intestine, and kidney.
Cell Membrane; Single-pass type I membrane protein .
BOSC23 cells were transiently trans-fected with an expression vector encoding either CEACAM19 (red curve) or an irrelevant protein (control transfectant). Binding of ab59717 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM19 transfected cells.
Spectral Confocal Microscopy of CHO cells using ab59717.
CHO cells were transiently transfected with an expression vector encoding CEACAM19. Binding of ab59717 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Kobayashi M et al. Carcinoembryonic antigen-related cell adhesion molecules as surrogate markers for EGFR inhibitor sensitivity in human lung adenocarcinoma. Br J Cancer107:1745-53 (2012).
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