Anti-CEACAM1 + CEACAM5抗体[4/3/17] (ab91213)
Key features and details
- Mouse monoclonal [4/3/17] to CEACAM1 + CEACAM5
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CEACAM1 + CEACAM5抗体[4/3/17]
参阅全部 CEACAM1 + CEACAM5 一抗 -
描述
小鼠单克隆抗体[4/3/17] to CEACAM1 + CEACAM5 -
宿主
Mouse -
特异性
Does not recognise CEACAM3, CEACAM6 or CEACAM8 -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Native Human protein expressed in mammalian cells
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阳性对照
- Transfected BOSC23 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
4/3/17 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91213于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 1.2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use 1.2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
CEACAM1 and CEACAM5 belong to the carcinoembryonic antigen (CEA) gene family. It is expressed on cells of epithelial and myeloid origin. CEACAM1 expression is downregulated in many tumors indicating a tumor - suppressive function. The antitumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of antiangiogenic molecules from the cells. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM. CEACAM5 is a cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is a homodimer. It is a receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. -
细胞定位
Isoforms A, B, C: Cell membrane; Single-pass type I membrane protein. Isoforms G, H, I: Secreted. -
数据库链接
- Entrez Gene: 1048 Human
- Entrez Gene: 634 Human
- Omim: 109770 Human
- Omim: 114890 Human
- SwissProt: P06731 Human
- SwissProt: P13688 Human
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别名
- Antigen CD66e antibody
- ATP dependent taurocolate carrier protein antibody
- BGP 1 antibody
see all
图片
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ab91213 at 1.2µg/106 cells in BOSC23 cells transiently transfected with an expression vector encoding either CEACAM1 (red curve) or a control protein (black curve). A PE conjugated secondary antibody was used.
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ab91213 at 1.2µg/106 cells in BOSC23 cells transiently transfected with an expression vector encoding either CEACAM5 (red curve) or a control protein (black curve). A PE conjugated secondary antibody was used.
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Specificity testing of ab91213. BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified ab91213 was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM1 and CEACAM5 expressing cells (red curves).
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab91213 尚未被引用在任何文献中。