Anti-CEACAM1抗体[YTH71.3] (ab104450)

概述

  • 产品名称Anti-CEACAM1抗体[YTH71.3]
    参阅全部 CEACAM1 一抗
  • 描述
    大鼠单克隆抗体[YTH71.3] to CEACAM1
  • 特异性Recognizes Human CEACAM1. CEACAM1 is expressed by Human neutrophils and cells in some cases of colon carcinoma. Reacts with transfectants containing BGPa, NCA and CGM1a which are CEA subfamily members.
  • 经测试应用适用于: IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Monkey
  • 免疫原

    ab104450 was raised against Human lymphocytes.

  • 阳性对照
    • Human Colon, Human Liver

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号YTH71.3
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab104450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 序列相似性Belongs to the immunoglobulin superfamily. CEA family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
  • Information by UniProt
  • 数据库链接
  • 形式There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
  • 别名
    • Antigen CD66 antibody
    • BGP 1 antibody
    • BGP antibody
    • BGP-1 antibody
    • BGPI antibody
    • Biliary glycoprotein 1 antibody
    • Biliary glycoprotein adhesion molecule antibody
    • Biliary glycoprotein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 1 antibody
    • CD66a antibody
    • CD66a antigen antibody
    • CEACAM1 antibody
    • CEAM1_HUMAN antibody
    • meconium antigen 100 antibody
    see all

Anti-CEACAM1 antibody [YTH71.3] 图像

  • Human Colon (formalin-fixed, paraffin-embedded) stained with ab104450 at 10 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Liver (formalin-fixed, paraffin-embedded) stained with ab104450 at 10 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-CEACAM1 antibody [YTH71.3] (ab104450)参考文献

ab104450 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Serum (mouse serum (with pancreatic tumor development))
Loading amount 50 µg
Specification mouse serum (with pancreatic tumor development)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
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Verified customer

提交于 May 24 2012

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Neither antibody has been tested for reactivity with rabbit CEACAM1, and the epitopes each antibody recognizes have not been mapped, so it is difficult to predict cross-reactivity with rabbit. If I needed to choo...

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Thank you for your inquiry. I was liaising with the laboratories to find out more about our pan-CEACAM- and CEACAM1-antibodies. Unfortunately, it turned out that we do not have any suitable antibody available: The immunogens of our CEACAM1 anti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"