概述

性能

应用

Our Abpromise guarantee covers the use of ab88129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

靶标

  • 功能
    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • 序列相似性
    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 翻译后修饰
    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Caudal type homeo box 2 antibody
    • Caudal type homeo box transcription factor 2 antibody
    • Caudal type homeobox 2 antibody
    • Caudal type homeobox protein 2 antibody
    • Caudal type homeobox transcription factor 2 antibody
    • Caudal-type homeobox protein 2 antibody
    • CDX 2 antibody
    • CDX 3 antibody
    • CDX-3 antibody
    • Cdx2 antibody
    • CDX2/AS antibody
    • CDX2_HUMAN antibody
    • CDX3 antibody
    • Homeobox protein CDX 2 antibody
    • Homeobox protein CDX-2 antibody
    see all

图片

  • All lanes : Anti-CDX2 antibody (ab88129) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • IHC image of CDX2 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88129, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

文献

This product has been referenced in:
  • Balahmar RM  et al. Identification and characterisation of NANOG+/ OCT-4high/SOX2+ doxorubicin-resistant stem-like cells from transformed trophoblastic cell lines. Oncotarget 9:7054-7065 (2018). Read more (PubMed: 29467949) »
  • Orsztynowicz M  et al. Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification. PLoS One 12:e0182398 (2017). Read more (PubMed: 28767705) »

See all 4 Publications for this product

客户评价及客户问答

Application
IHC - Wholemount
Sample
Cow Embryo (fetal membrane)
Specification
fetal membrane
Username

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Verified customer

提交于 Nov 25 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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