Anti-CDX2抗体(ab48009)

概述

性能

应用

Our Abpromise guarantee covers the use of ab48009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/4000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).

靶标

  • 功能
    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • 序列相似性
    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 翻译后修饰
    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Caudal type homeo box 2 antibody
    • Caudal type homeo box transcription factor 2 antibody
    • Caudal type homeobox 2 antibody
    • Caudal type homeobox protein 2 antibody
    • Caudal type homeobox transcription factor 2 antibody
    • Caudal-type homeobox protein 2 antibody
    • CDX 2 antibody
    • CDX 3 antibody
    • CDX-3 antibody
    • Cdx2 antibody
    • CDX2/AS antibody
    • CDX2_HUMAN antibody
    • CDX3 antibody
    • Homeobox protein CDX 2 antibody
    • Homeobox protein CDX-2 antibody
    see all

Anti-CDX2 antibody 图像

  • Anti-CDX2 antibody (ab48009) at 1 µg/ml + Human Colon lysate (35µg protein in RIPA buffer).

    Predicted band size : 34 kDa

实验方案

Anti-CDX2 antibody (ab48009)参考文献

ab48009 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x Dako Target Retrival Solution in Water
Permeabilization
Yes - 0.5% Tween-20 in PBS
Specification
Colon
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
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提交于 Jun 22 2017

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Since ab48009 has not been testedwith mouse...

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