Anti-CDT2/RAMP抗体(ab72264)
Key features and details
- Rabbit polyclonal to CDT2/RAMP
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CDT2/RAMP抗体
参阅全部 CDT2/RAMP 一抗 -
描述
兔多克隆抗体to CDT2/RAMP -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human CDT2/RAMP aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9NZJ0 -
阳性对照
- WB: Jurkat, HeLa and HEK-293T whole cell lysate. IP: Jurkat whole cell lysate. IHC-P: Human prostate carcinoma. Mouse plasmacytoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72264于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 91 kDa (predicted molecular weight: 79 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 91 kDa (predicted molecular weight: 79 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. -
组织特异性
Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the WD repeat cdt2 family.
Contains 7 WD repeats. -
发展阶段
Expressed in all fetal tissues examined, included brain, lung, liver, and kidney. -
翻译后修饰
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C).
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Nucleus membrane. Cytoplasm > cytoskeleton > centrosome. Nuclear matrix-associated protein. Translocates from the interphase nucleus to the metaphase cytoplasm during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 51514 Human
- Entrez Gene: 76843 Mouse
- Omim: 610617 Human
- SwissProt: Q9NZJ0 Human
- SwissProt: Q3TLR7 Mouse
- Unigene: 656473 Human
- Unigene: 189102 Mouse
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别名
- Lethal(2) denticleless protein homolog antibody
- CDW1 antibody
- DCAF2 antibody
see all
图片
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All lanes : Anti-CDT2/RAMP antibody (ab72264) at 0.04 µg/ml
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 79 kDa
Exposure time: 75 secondsLysates prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CDT2/RAMP with ab72264 at 1/1000 (0.2 µg/ml). Detection: DAB.
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Detection of human CDT2/RAMP by western blot of immunoprecipitates.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer.
Antibodies: ab72264 used for IP at 6 µg per reaction, lanes 1 and 2, lane 3 is control IgG. DTL/CDT2 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated CDT2/RAMP, ab72264 was used at 0.1 µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse plasmacytoma tissue labelling CDT2/RAMP with ab72264 at 1/200 (1 µg/ml). Detection: DAB.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab72264 被引用在 8 文献中.
- Luo Y et al. DTL Is a Prognostic Biomarker and Promotes Bladder Cancer Progression through Regulating the AKT/mTOR axis. Oxid Med Cell Longev 2022:3369858 (2022). PubMed: 35103094
- Li Z et al. Role of DTL in Hepatocellular Carcinoma and Its Impact on the Tumor Microenvironment. Front Immunol 13:834606 (2022). PubMed: 35392073
- Ma Y et al. Cdk2 suppresses IL-23 expression and the onset of severe acute pancreatitis. Immun Inflamm Dis 10:e631 (2022). PubMed: 35634959
- Nathans JF et al. Cell cycle inertia underlies a bifurcation in cell fates after DNA damage. Sci Adv 7:N/A (2021). PubMed: 33523889
- Liu S et al. Overexpression of DTL enhances cell motility and promotes tumor metastasis in cervical adenocarcinoma by inducing RAC1-JNK-FOXO1 axis. Cell Death Dis 12:929 (2021). PubMed: 34635635
- Hou L et al. Restriction of hepatitis B virus replication by c-Abl-induced proteasomal degradation of the viral polymerase. Sci Adv 5:eaau7130 (2019). PubMed: 30775435
- Jang SM et al. The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin. Nat Commun 9:2782 (2018). PubMed: 30018425
- Jascur T et al. N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) Triggers MSH2 and Cdt2 Protein-dependent Degradation of the Cell Cycle and Mismatch Repair (MMR) Inhibitor Protein p21Waf1/Cip1. J Biol Chem 286:29531-9 (2011). WB ; Human . PubMed: 21725088