概述

  • 产品名称Anti-CDT1抗体
    参阅全部 CDT1 一抗
  • 描述
    山羊多克隆抗体to CDT1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    ARLAHQTRAEEGL

    by a Cysteine residue linker, corresponding to C terminal amino acids 534-546 of Human CDT1

  • 阳性对照
    • Human brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab77091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/64000 in peptide ELISA assay.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
    • 序列相似性Belongs to the Cdt1 family.
    • 发展阶段Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
    • 结构域The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
    • 翻译后修饰Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. The interaction with GMNN protects it against ubiquitination.
      Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDT 1 antibody
      • cdt1 antibody
      • CDT1_HUMAN antibody
      • Chromatin licensing and DNA replication factor 1 antibody
      • DNA replication factor antibody
      • DNA replication factor Cdt1 antibody
      • Double parked antibody
      • Double parked Drosophila homolog of antibody
      • Double parked homolog antibody
      • DUP antibody
      • Retroviral integration site 1 antibody
      • Retroviral integration site 2 antibody
      • Retroviral integration site1 antibody
      • Retroviral integration site2 antibody
      • RIS 2 antibody
      • RIS2 antibody
      see all

    Anti-CDT1 antibody 图像

    • Anti-CDT1 antibody (ab77091) at 1 µg/ml + Human brain lysate at 35 µg
      Developed using the ECL technique

      Predicted band size : 63 kDa
      Observed band size : 63 kDa

    Anti-CDT1 antibody (ab77091)参考文献

    ab77091 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77091.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"