Anti-CDT1/DUP抗体(ab70829)
Key features and details
- Rabbit polyclonal to CDT1/DUP
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CDT1/DUP抗体
参阅全部 CDT1/DUP 一抗 -
描述
兔多克隆抗体to CDT1/DUP -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee -
免疫原
Synthetic peptide corresponding to Human CDT1/DUP (C terminal).
Database link: NP_112190.1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab70829 was affinity purified using an epitope specific to CDT1/DUP immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70829于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
1/1000 - 1/2500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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说明 |
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IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/2500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
靶标
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功能
Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene. -
序列相似性
Belongs to the Cdt1 family. -
发展阶段
Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases. -
结构域
The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination. -
翻译后修饰
Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. The interaction with GMNN protects it against ubiquitination.
Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 81620 Human
- Omim: 605525 Human
- SwissProt: Q9H211 Human
- Unigene: 122908 Human
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别名
- CDT 1 antibody
- cdt1 antibody
- CDT1_HUMAN antibody
see all
图片
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All lanes : Anti-CDT1/DUP antibody (ab70829) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 3 minutesSecondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human islet cell carcinoma tissue labelling CDT1/DUP with ab70829 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of CDT1/DUP by Western Blot of Immunprecipitate.
ab70829 at 1µg/ml staining CDT1/DUP in HeLa whole cell lysate immunoprecipitated using ab70829 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).Secondary antibody anti-rabbit HRP (ab6721)
Detection: Chemiluminescence with exposure time of 3 minutes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab70829 被引用在 10 文献中.
- Simoneschi D et al. CRL4AMBRA1 is a master regulator of D-type cyclins. Nature 592:789-793 (2021). PubMed: 33854235
- Rose JT et al. Inhibition of the RUNX1-CBFß transcription factor complex compromises mammary epithelial cell identity: a phenotype potentially stabilized by mitotic gene bookmarking. Oncotarget 11:2512-2530 (2020). PubMed: 32655837
- Hernández-Pérez S et al. USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication. Mol Oncol N/A:N/A (2016). WB ; Human . PubMed: 27296872
- Grandy RA et al. Genome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon Differentiation. Mol Cell Biol 36:615-27 (2015). WB ; Human . PubMed: 26644406
- Hayashi A et al. PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells. Methods Mol Biol 1170:367-82 (2014). PubMed: 24906324
- Clijsters L & Wolthuis R PIP-box-mediated degradation prohibits re-accumulation of Cdc6 during S phase. J Cell Sci 127:1336-45 (2014). PubMed: 24434580
- Yang YL et al. Lung tumourigenesis in a conditional Cul4A transgenic mouse model. J Pathol 233:113-23 (2014). IHC-P . PubMed: 24648314
- Maya-Mendoza A et al. Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state. Cell Death Dis 5:e1351 (2014). WB ; Human . PubMed: 25058425
- Clijsters L et al. The spindle checkpoint, APC/C(Cdc20), and APC/C(Cdh1) play distinct roles in connecting mitosis to S phase. J Cell Biol 201:1013-26 (2013). Human . PubMed: 23775192
- Rossetto CC et al. Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells. PLoS Pathog 9:e1003366 (2013). ChIP ; Human . PubMed: 23717203