概述

  • 产品名称Anti-CDS1抗体
    参阅全部 CDS1 一抗
  • 描述
    兔多克隆抗体to CDS1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (VVFGFIAAYV LSKYQYFVCP VEYRSDVNSF VTECEPSELF QLQTYSLPPF) of Human CDS1 (NP_001254).

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Provides CDP-diacylglycerol an important precursor for the synthesis of phosphatidylinositol (PtdIns), phosphatidylglycerol, and cardiolipin. Overexpression may amplify cellular signaling responses from cytokines. May also play an important role in the signal transduction mechanism of retina and neural cells.
  • 组织特异性Expressed in adult tissues such as placenta, brain, small intestine, ovary, testis and prostate. Highly expressed in fetal kidney, lung and brain. Lower level in fetal liver.
  • 通路Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.
  • 序列相似性Belongs to the CDS family.
  • 细胞定位Endoplasmic reticulum membrane. Cytoplasmic aspect of the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDP DAG synthase 1 antibody
    • CDP DG synthetase 1 antibody
    • CDP diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 antibody
    • CDP diacylglycerol synthase 1 antibody
    • CDP diglyceride pyrophosphorylase 1 antibody
    • CDP diglyceride synthetase 1 antibody
    • CDP-DAG synthase 1 antibody
    • CDP-DG synthase 1 antibody
    • CDP-diacylglycerol synthase 1 antibody
    • CDP-diglyceride pyrophosphorylase 1 antibody
    • CDP-diglyceride synthase 1 antibody
    • CDS 1 antibody
    • CDS antibody
    • CDS1 antibody
    • CDS1_HUMAN antibody
    • CTP:phosphatidate cytidylyltransferase 1 antibody
    • Phosphatidate cytidylyltransferase 1 antibody
    see all

Anti-CDS1 antibody 图像

  • Anti-CDS1 antibody (ab84019) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Anti-CDS1 antibody (ab84019)参考文献

This product has been referenced in:
  • Croston TL  et al. Evaluation of the cardiolipin biosynthetic pathway and its interactions in the diabetic heart. Life Sci 93:313-22 (2013). Read more (PubMed: 23872101) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Sample Rat Tissue lysate - other (Heart)
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 50 µg
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Evelyn Gomez Espinosa

Verified customer

提交于 Sep 04 2015

Thanks for your call today and I'm sorry that this antibody did not work as expected. I have issued a discount code, which will be activated by submitting an Abreview of ab84019 with rat samples. You canredeem this discount code for any primary antibod...

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Thank you again for your call yesterday, and also for your patience while I have looked through some literature as well as through the Western blot images.

I do have a couple of additional questions to clarify this data, as I haven't worked w...

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Thanks for your call today and for letting us know about the trouble with ab84019.

As we discussed, please forward me an image of your blot when you get a chance. I'll check the literature and our protocol for this antibody and see if there '...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 mouse fibroblast cell line)
Loading amount 40 µg
Specification NIH 3T3 mouse fibroblast cell line
Treatment 48 hs-serum deprived
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. GERMAN GIL

Verified customer

提交于 Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"