Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
A region within synthetic peptide: MLAENLVEEF EMKEDEPWYD HQDLQQDLQL AAELGKTLLD RNTELEDSVQ, corresponding to N terminal amino acids 1-50 of Human CDR2
- HepG2 cell lysate and human liver tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use a concentration of 4 - 8 µg/ml.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Belongs to the CDR2 family.
Information by UniProt
- AA617262 antibody
- CDR 2 antibody
- CDR 62 antibody
Western blot - Anti-CDR2 antibody (ab65531)
Anti-CDR2 antibody (ab65531) at 5 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDR2 antibody (ab65531)
ab65531 at 4µg/ml staining CDR2 in hepatocytes of human liver (indicated by arrows) by Immunohistochemistry, Paraffin-embedded tissue.
has not yet been referenced specifically in any publications.
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