This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-CDON / Cdo抗体(ab34189)

概述

性能

应用

  • 应用说明
    This antibody gave a positive result in ELISA against the immunizing peptide (ab34188).
    Not yet tested in other applications.

  • 靶标

    Anti-CDON / Cdo antibody 图像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • ICC/IF image of ab34189 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34189, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.  However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.

    Anti-CDON / Cdo antibody (ab34189)参考文献

    ab34189 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (KM-H2)
    Specification KM-H2
    Preparation Cell harvesting/tissue preparation method: Asynchronous KM-H2 were harvested and labeled by indirect immunofluorescence
    Sample buffer: PBS + 5% FBS
    Permeabilization No
    Gating Strategy Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive.
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Dec 16 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"