概述

  • 产品名称Anti-CDKN3抗体
    参阅全部 CDKN3 一抗
  • 描述
    兔多克隆抗体to CDKN3
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 67-116 (

    LKSYGIQDVFVFCTRGELSKYRVPNLLDLYQQYGIVTHHHPIPDGGTPDI

    ) of Mouse CDKN3 (NP_082498).

  • 阳性对照
    • Mouse heart lysate IF/ICC: NIH 3T3 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab118702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.
  • 疾病相关Defects in CDKN3 are found in patients with hepatocellular carcinoma (HCC) [MIM:114550].
  • 序列相似性Belongs to the protein-tyrosine phosphatase family.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDI1 antibody
    • Cdk associated protein phosphatase antibody
    • CDK2 associated dual specificity phosphatase antibody
    • CDK2-associated dual-specificity phosphatase antibody
    • CDKN3 antibody
    • CDKN3_HUMAN antibody
    • CIP2 antibody
    • Cyclin dependent kinase inhibitor 3 antibody
    • Cyclin dependent kinase interacting protein 2 antibody
    • Cyclin dependent kinase interactor 1 antibody
    • Cyclin-dependent kinase inhibitor 3 antibody
    • Cyclin-dependent kinase interactor 1 antibody
    • Cyclin-dependent kinase-interacting protein 2 antibody
    • FLJ25787 antibody
    • KAP antibody
    • KAP1 antibody
    • Kinase associated phosphatase antibody
    • Kinase-associated phosphatase antibody
    • MGC70625 antibody
    • OTTHUMP00000178991 antibody
    see all

Anti-CDKN3 antibody 图像

  • ab118702 stained NIH 3T3 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118702 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

     

  • Anti-CDKN3 antibody (ab118702) at 1 µg/ml + Mouse heart lysate at 10 µg

    Predicted band size : 24 kDa
    Gel concentration: 12%

Anti-CDKN3 antibody (ab118702)参考文献

This product has been referenced in:
  • Deng M  et al. Silencing cyclin-dependent kinase inhibitor 3 inhibits the migration of breast cancer cell lines. Mol Med Rep 14:1523-30 (2016). WB ; Human . Read more (PubMed: 27314680) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I am sorry that the other CDNK3 products have not been acceptable in your assays and that you have been experiencing issues when using ab119242. This product would be able to detect endogenous CDKN3, the cell line chosen ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"