概述

  • 产品名称Anti-CDKN2A/p19ARF抗体[5-C3]
    参阅全部 CDKN2A/p19ARF 一抗
  • 描述
    大鼠单克隆抗体[5-C3] to CDKN2A/p19ARF
  • 经测试应用适用于: WB, IP, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Hamster, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse p19ARF.

  • 阳性对照
    • 3T3 cell lystate positive control.

性能

应用

Our Abpromise guarantee covers the use of ab26696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/1000.
IHC-FoFr Use a concentration of 5 µg/ml.

靶标

  • 相关性The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ARF antibody
    • CDKN2A antibody
    • Cyclin dependent kinase inhibitor 2A antibody
    • INK4 antibody
    • MTS1 antibody
    • P19 antibody
    see all

Anti-CDKN2A/p19ARF antibody [5-C3] 图像

  • All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
    Lane 2 : MEF total cell lysate

    Secondary
    anti-rat IgG-HRP

    Predicted band size : 17 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.
  • Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).

Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)参考文献

This product has been referenced in:
  • Bartesaghi S  et al. Inhibition of oxidative metabolism leads to p53 genetic inactivation and transformation in neural stem cells. Proc Natl Acad Sci U S A 112:1059-64 (2015). WB . Read more (PubMed: 25583481) »
  • Shai A  et al. TP53 Silencing Bypasses Growth Arrest of BRAFV600E-Induced Lung Tumor Cells in a Two-Switch Model of Lung Tumorigenesis. Cancer Res 75:3167-80 (2015). WB . Read more (PubMed: 26001956) »

See all 12 Publications for this product

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