The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml.
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted Lane 2 : MEF total cell lysate
Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.
Lee CK et al. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene35:1988-95 (2016).
Read more (PubMed: 26212014) »
Bartesaghi S et al. Inhibition of oxidative metabolism leads to p53 genetic inactivation and transformation in neural stem cells. Proc Natl Acad Sci U S A112:1059-64 (2015).
Read more (PubMed: 25583481) »