RabMAb

Anti-CDKN2A/p16INK4a抗体[EPR1473] (ab108349)

概述

  • 产品名称Anti-CDKN2A/p16INK4a抗体[EPR1473]
    参阅全部 CDKN2A/p16INK4a 一抗
  • 描述
    兔单克隆抗体[EPR1473] to CDKN2A/p16INK4a
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDKN2A/p16INK4a aa 100 to the C-terminus (C terminal).

  • 阳性对照
    • WB: HeLa, HEK293, 293T, and Saos-2 cell lysates. IHC-P: Human cervical carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HEK293 and HeLa cells. IP: HeLa cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. A trial size is available to purchase for this antibody.

     

    Alternative versions available:

    Anti-CDKN2A/p16INK4a antibody (Alexa Fluor® 488) [EPR1473] (ab192053)

    Anti-CDKN2A/p16INK4a antibody (Alexa Fluor® 647) [EPR1473] (ab192054)

    Anti-CDKN2A/p16INK4a antibody (HRP) [EPR1473] (ab192080)

    Anti-CDKN2A/p16INK4a antibody (Phycoerythrin) [EPR1473] (ab209579)

性能

应用

Our Abpromise guarantee covers the use of ab108349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 17 kDa.

For unpurified use at 1/1000 - 1/10000.

IP 1/30.

For unpurified use at 1/10 - 1/100.

IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/250 - 1/500.

Flow Cyt 1/270.

For unpurified use at 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/270.

For unpurified use at 1/100 - 1/250.

靶标

  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 形式There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
  • 别名
    • CCM2 antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
    • Cyclin Dependent Kinase Inhibitor 2A antibody
    • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
    • Cyclin dependent kinase inhibitor p16 antibody
    • INK4 antibody
    • INK4A antibody
    • MLM antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • P16INK4 antibody
    • p16INK4a antibody
    • p19 antibody
    • p19Arf antibody
    • TP16 antibody
    see all

Anti-CDKN2A/p16INK4a antibody [EPR1473] 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling CDKN2A/p16INK4a with purified ab108349 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CDKN2A/p16INK4a with unpurified ab108349 at a dilution of 1/100.

  • Flow Cytometry analysis of HEK293 cells labelling CDKN2A/p16INK4a with purified ab108349 at 1/270 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • All lanes : Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab108349) at 1/2000 dilution (purified)

    Lane 1 : HEK293 cell lysate
    Lane 2 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 17 kDa
    Observed band size : 17 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab108349) at 1/2000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 17 kDa
    Observed band size : 17 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab108349) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 17 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling CDKN2A/p16INK4a with unpurified ab108349 at a dilution of 1/250.

  • Overlay histogram showing HEK293 cells stained with unpurified ab108349 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108349, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab108349 (purified) at 1/30 immunoprecipitating CDKN2A/p16INK4a in HeLa cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab108349)参考文献

This product has been referenced in:
  • Idriss MH  et al. Orthokeratotic Bowen disease: a histopathologic, immunohistochemical and molecular study. J Cutan Pathol 43:24-31 (2016). IHC-P ; Human . Read more (PubMed: 26272630) »
  • Liang L  et al. Assessment of the Utility of PAX8 Immunohistochemical Stain in Diagnosing Endocervical Glandular Lesions. Arch Pathol Lab Med 140:148-52 (2016). IHC-P ; Human . Read more (PubMed: 26910219) »

See all 22 Publications for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (Purified recombinant p16 (overexpressed in HEK293))
Specification Purified recombinant p16 (overexpressed in HEK293)
Type Sandwich (Detection)
Blocking step SuperBlock PBS Pierce #37580 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 28 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (B16F10 melanoma cancer implanted)
Specification B16F10 melanoma cancer implanted
Fixative 4% Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: autoclaved for 10min at 121 Celcius (10mM Sodium Citrate, pH 6.0)
Permeabilization Yes - 0.1% SDS
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

提交于 Mar 15 2013

Thank you for contacting us.

We do not provide Abtrial code for tested species and applications. Please check and explain this to customer;

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"