产品名称 Anti-CDKN2A/p16INK4a抗体 参阅全部 CDKN2A/p16INK4a 一抗
ICC/IF, WB more details
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human CDKN2A/p16INK4a .
This antibody gave a positive signal in both HeLa and HEK293 whole cell lysates.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
细胞定位 Cytoplasmic and Nuclear
形式 There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
CDK4 inhibitor p16 INK4 antibody
Anti-CDKN2A/p16INK4a antibody 图像
Western blot - CDKN2A/p16INK4a antibody (ab94619)
All lanes : Anti-CDKN2A/p16INK4a antibody (ab94619) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 17 kDa Observed band size : 17 kDa Exposure time : 4 minutes
Immunocytochemistry/ Immunofluorescence - CDKN2A/p16INK4a antibody (ab94619)
ICC/IF image of ab94619 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94619, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-CDKN2A/p16INK4a antibody (ab94619)参考文献
has not yet been referenced specifically in any publications.
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