Anti-CDKN2A/p16INK4a抗体(ab6546)

概述

  • 产品名称Anti-CDKN2A/p16INK4a抗体
    参阅全部 CDKN2A/p16INK4a 一抗
  • 描述
    兔多克隆抗体to CDKN2A/p16INK4a
  • 特异性Cross reactivity may occur with p15 when immunoprecipitating in vitro translated product but not in immunoblots. Potentially cross reacts with p15.5.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    human p16 protein (missing the first 7 amino acids)

  • 阳性对照
    • Saos-2 lysate
  • 常规说明p16 (also known as p16 -INK4a, p16-MTS1, inhibitor of CDK4), is the product of the CDKN2 gene. It inhibits the progression of cell cycle through the G1 phase, by competing with D type cyclins to bind to CDK4 and CDK6. p16INK4/CDKN2is a candidate tumor suppressor, whose gene is frequently deleted or mutated in diverse types of cancer.

性能

应用

Our Abpromise guarantee covers the use of ab6546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 17 kDa.
    This antisera is also suitable for immmunoprecipitation of in vitro translated protein and cell lysates (Saos-2, 3T3-L1 weakly) and will co-precipitate associated proteins including p15 and potentially p15.5.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 细胞定位Cytoplasmic and Nuclear
    • 数据库链接
    • 形式There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
    • 别名
      • CCM2 antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
      • Cyclin Dependent Kinase Inhibitor 2A antibody
      • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
      • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
      • Cyclin dependent kinase inhibitor p16 antibody
      • INK4 antibody
      • INK4A antibody
      • MLM antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • P16INK4 antibody
      • p16INK4a antibody
      • p19 antibody
      • p19Arf antibody
      • TP16 antibody
      see all

    Anti-CDKN2A/p16INK4a antibody (ab6546)参考文献

    ab6546 has not yet been referenced specifically in any publications.

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    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below;

    http://www.abcam.com/ps/pdf/protocols/Sandwich%20ELISA.pdf

    http://www.abca...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"