Anti-CDKN2A/p16INK4a抗体[2D9A12] (ab54210)

概述

  • 产品名称
    Anti-CDKN2A/p16INK4a抗体[2D9A12]
    参阅全部 CDKN2A/p16INK4a 一抗
  • 描述
    小鼠单克隆抗体[2D9A12] to CDKN2A/p16INK4a
  • 经测试应用
    适用于: Flow Cyt, ELISA, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to Human CDKN2A/p16INK4a.
    Database link: P42771

  • 阳性对照
    • Rat liver tissue; Human brain tissue; Human brain tumor tissue; PFA/Tween HeLa cells.
  • 常规说明

    We have received positive and negative feedback from customers for ICC/IF. ICC/IF is not a batch-tested application for this antibody and we are not able to cover it under our Abpromise guarantee.

性能

应用

Our Abpromise guarantee covers the use of ab54210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ELISA 1/10000.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommended antigen retrieval: Place slides in a special glass slide holder and fill in the rack with slides to ensure even heating. Rinse rack in 1L of Citrate buffer in ordinary pressure cooker,(0.01M,pH6.0) to immerse all slides. Cover the cap and heat for 15-20 minutes on electric cooker until water boils. Begin timing, only 2 minutes in high-pressure Remove the pressure cooker, Allow it to cool(Cool the slides at room temperature) Rinse the slides in PBS for 5 minutes
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 细胞定位
      Cytoplasmic and Nuclear
    • 数据库链接
    • 形式
      There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
    • 别名
      • CCM2 antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
      • Cyclin Dependent Kinase Inhibitor 2A antibody
      • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
      • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
      • Cyclin dependent kinase inhibitor p16 antibody
      • INK4 antibody
      • INK4A antibody
      • MLM antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • P16INK4 antibody
      • p16INK4a antibody
      • p19 antibody
      • p19Arf antibody
      • TP16 antibody
      see all

    Anti-CDKN2A/p16INK4a antibody [2D9A12] 图像

    •  ab54210 (1:500) staining p16 INK4a in human brain tumor tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    • ab54210 staining CDKN2A/p16INK4a in Human cervix and ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000 in Dako diluent) for 20 minutes at 25°C. An undiluted HRP-conjugated Mouse polymer was used as the secondary antibody.

      See Abreview

    • ab54210 staining CDKN2A/p16INK4a in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 1 hour at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 1% NGS/TBS) for 20 hours at 4°C. Ab6785 (1/800) was used as the secondary antibody.

      See Abreview

    • ab54210 (1:500) staining p16 INK4a in rat liver tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    • Overlay histogram showing HeLa cells stained with ab54210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54210, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    •  ab54210 (1:500) staining p16 INK4a in human brain tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)参考文献

    This product has been referenced in:
    • Tanaka T  et al. The efficacy of the cyclin-dependent kinase 4/6 inhibitor in endometrial cancer. PLoS One 12:e0177019 (2017). WB, IHC-P ; Human . Read more (PubMed: 28472136) »
    • Mirkheshti N  et al. Dual targeting of androgen receptor and mTORC1 by salinomycin in prostate cancer. Oncotarget 7:62240-62254 (2016). Read more (PubMed: 27557496) »

    See all 24 Publications for this product

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    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Inguinal Adipose Tissue)
    Gel Running Conditions
    Reduced Denaturing (2-20% Precast Gel, 100V for 1 hr 20 min to run)
    Loading amount
    40 µg
    Specification
    Inguinal Adipose Tissue
    Blocking step
    SuperBlock T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 13 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain (medulloblastoma preneoplastic lesions))
    Permeabilization
    Yes - 0.3% Triton
    Specification
    Brain (medulloblastoma preneoplastic lesions)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Lukas Tamayo

    Verified customer

    提交于 Jun 20 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (brain section)
    Permeabilization
    Yes - 0.8% Triton-100 in PBS
    Specification
    brain section
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative
    Paraformaldehyde
    Username

    Peisu Zhang

    Verified customer

    提交于 Mar 15 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (vascular smooth muscle cell)
    Permeabilization
    Yes - NP40
    Specification
    vascular smooth muscle cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jan 06 2016

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (15%gel)
    Sample
    Mouse Tissue lysate - whole (hippocampus)
    Specification
    hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 10 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (heart (left ventricle))
    Gel Running Conditions
    Reduced Denaturing (15% poly-acrylamide)
    Loading amount
    20 µg
    Specification
    heart (left ventricle)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 07 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
    Permeabilization
    Yes - TBST
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 16 2010

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver, spleen)
    Loading amount
    80 µg
    Specification
    Liver, spleen
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 20 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (ovarian carcinoma & cervix)
    Specification
    ovarian carcinoma & cervix
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, ER1 (Leica)
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    提交于 Dec 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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