Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CDKN2A/p16INK4a antibody [2D9A12]

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization Yes - TBST
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Other product details

Dilution 1/100
Incubation time 20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% NGS/TBS

Secondary antibody

Dilution 1/800

Additional data

Additional Notes Heat mediated antigen retrieval: microwave, 10 minutes, sub-boiling, citrate buffer pH 6.0. The nuclei are counterstained with propidium iodide (red).
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提交于 Feb 16 2010