Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CDKN2A/p16INK4a antibody [2D9A12]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (ovarian carcinoma & cervix)
Specification ovarian carcinoma & cervix
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, ER1 (Leica)
Permeabilization No

Other product details

Dilution 1/4000
Incubation time 20 minute(s) · Temperature: 25°C · Diluent: Dako Diluent, background reducing agents

Secondary antibody

Name Non-Abcam antibody was used: Post-polymer (Leica Refine)
Host species: Mouse
Clonality: Monoclonal
Conjugation: HRP polymer

Additional data

Additional Notes Use Refine Detection Kit (Leica) and automated staining on BondMax.
Antigen retrieval at 100C for 20 minutes in citrate buffer pH6.0
Username

Abcam user community

Verified customer

提交于 Dec 08 2008