Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CDKN2A/p16INK4a antibody [2D9A12]

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (ovarian carcinoma & cervix)
Specification
ovarian carcinoma & cervix
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, ER1 (Leica)
Permeabilization
No

Other product details

Dilution
1/4000
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako Diluent, background reducing agents

Secondary antibody

Name
Non-Abcam antibody was used: Post-polymer (Leica Refine)
Host species: Mouse
Clonality: Monoclonal
Conjugation: HRP polymer

Additional data

Additional Notes
Use Refine Detection Kit (Leica) and automated staining on BondMax.
Antigen retrieval at 100C for 20 minutes in citrate buffer pH6.0
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提交于 Dec 08 2008

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