Anti-CDKN1A interacting zinc finger蛋白1/CIZ1抗体(ab102013)
Key features and details
- Rabbit polyclonal to CDKN1A interacting zinc finger protein 1/CIZ1
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CDKN1A interacting zinc finger蛋白1/CIZ1抗体
参阅全部 CDKN1A interacting zinc finger protein 1/CIZ1 一抗 -
描述
兔多克隆抗体to CDKN1A interacting zinc finger蛋白1/CIZ1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CDKN1A interacting zinc finger protein 1/CIZ1 aa 200-460.
Database link: NP_036259 -
阳性对照
- A549, HeLa, HepG2, HCT116 IP: HeLa whole cell lysate. IHC-P: ES2 xenograft tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102013于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 91 kDa.
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IHC-P |
1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended.
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说明 |
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ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 91 kDa. |
IHC-P
1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended. |
靶标
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功能
May regulate the subcellular localization of CIP/WAF1. -
疾病相关
Defects in CIZ1 may be a cause of adult onset primary cervical dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Cervical dystonia or spasmodic torticollis, the most common form of focal dystonia, is characterized by involuntary contractions of the neck muscles, which produce abnormal posturing of the head upon the trunk. -
序列相似性
Contains 1 matrin-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 25792 Human
- Omim: 116899 Human
- SwissProt: Q9ULV3 Human
- Unigene: 212395 Human
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别名
- CDKI antibody
- CDKN1A-interacting zinc finger protein 1 antibody
- Cip1-interacting zinc finger protein antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded ES2 xenograft using ab102013 at 1/500 dilution.
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Paraformaldehyde-fixed HeLa(human epithelial cell line from cervix adenocarcinoma) cells labeling CDKN1A interacting zinc finger protein 1/CIZ1 using ab102013 at 1/200 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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CDKN1A interacting zinc finger protein 1/CIZ1 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 μg of ab102013. Western blot was performed from the immunoprecipitate using ab102013 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 μg (Input).
Lane 2: Control IgG IP in HeLa whole cell lysate.
Lane 3: ab102013 IP in HeLa whole cell lysate.
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Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 91 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab102013 被引用在 5 文献中.
- Guo M & Zhang X LncRNA MSTO2P promotes colorectal cancer progression through epigenetically silencing CDKN1A mediated by EZH2. World J Surg Oncol 20:95 (2022). PubMed: 35346226
- Shao Y et al. Ubiquitin-like protein FAT10 promotes renal fibrosis by stabilizing USP7 to prolong CHK1-mediated G2/M arrest in renal tubular epithelial cells. Aging (Albany NY) 14:7527-7546 (2022). PubMed: 36152057
- Zhou X et al. CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma. Oncol Lett 15:183-188 (2018). WB, IHC-P ; Human . PubMed: 29285191
- Gang X et al. Treatment effect of CDKN1A on rheumatoid arthritis by mediating proliferation and invasion of fibroblast-like synoviocytes cells. Clin Exp Immunol N/A:N/A (2018). PubMed: 29920650
- Wang DQ et al. Ciz1 is a novel predictor of survival in human colon cancer. Exp Biol Med (Maywood) N/A:N/A (2014). PubMed: 24928862