RabMAb

Anti-Cdk9抗体[EP3118] (ab75848)

概述

  • 产品名称Anti-Cdk9抗体[EP3118]
    参阅全部 Cdk9 一抗
  • 描述
    兔单克隆抗体[EP3118] to Cdk9
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in human Cdk9

  • 阳性对照
    • HT-29, HeLa and 293T cell lysates. Human breast carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab75848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/2000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

    Is unsuitable for Flow Cyt, ICC or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C-2K antibody
      • CDC2 related kinase antibody
      • CDC2L4 antibody
      • Cdk 9 antibody
      • Cdk9 antibody
      • CDK9_HUMAN antibody
      • Cell division cycle 2-like protein kinase 4 antibody
      • Cell division protein kinase 9 antibody
      • CTK1 antibody
      • Cyclin dependent kinase 9 antibody
      • Cyclin-dependent kinase 9 antibody
      • PITALRE antibody
      • Serine/threonine-protein kinase PITALRE antibody
      • TAK antibody
      • Tat associated kinase complex catalytic subunit antibody
      see all

    Anti-Cdk9 antibody [EP3118] 图像

    • ab75848 at 1/100 dilution staining Cdk9 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.
    • All lanes : Anti-Cdk9 antibody [EP3118] (ab75848) at 1/2000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa

    Anti-Cdk9 antibody [EP3118] (ab75848)参考文献

    ab75848 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab75848.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"