概述

  • 产品名称Anti-Cdk8抗体
    参阅全部 Cdk8 一抗
  • 描述
    兔多克隆抗体to Cdk8
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide (Human) derived from the N-terminal of Cdk8.

  • 阳性对照
    • Extracts from 293 cells.

性能

应用

Our Abpromise guarantee covers the use of ab64940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cdk8 antibody
    • CDK8 protein kinase antibody
    • CDK8_HUMAN antibody
    • Cell Division Kinase 8 antibody
    • Cell division protein kinase 8 antibody
    • Cyclin Dependent kinase 8 antibody
    • Cyclin-dependent kinase 8 antibody
    • K35 antibody
    • Mediator complex subunit cdk8 antibody
    • Mediator of RNA polymerase II transcription subunit cdk8 antibody
    • Protein kinase K35 antibody
    see all

Anti-Cdk8 antibody 图像

  • All lanes : Anti-Cdk8 antibody (ab64940) at 1/500 dilution

    Lane 1 : extracts from 293 cells (5-30µg total protein)
    Lane 2 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.

Anti-Cdk8 antibody (ab64940)参考文献

ab64940 has not yet been referenced specifically in any publications.

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