概述

  • 产品名称Anti-Cdk8抗体
    参阅全部 Cdk8 一抗
  • 描述
    兔多克隆抗体to Cdk8
  • 经测试应用适用于: IP, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis , Medaka fish
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 370-420 of Human Cdk8 (P49336).

  • 阳性对照
    • HeLa cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab115155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
    • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • cdk8 antibody
      • CDK8 protein kinase antibody
      • CDK8_HUMAN antibody
      • Cell Division Kinase 8 antibody
      • Cell division protein kinase 8 antibody
      • Cyclin Dependent kinase 8 antibody
      • Cyclin-dependent kinase 8 antibody
      • K35 antibody
      • Mediator complex subunit cdk8 antibody
      • Mediator of RNA polymerase II transcription subunit cdk8 antibody
      • Protein kinase K35 antibody
      see all

    Anti-Cdk8 antibody 图像

    • Immunoprecipitation of Cdk8 (3 µg of antibody per mg of HeLa cells lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdk8 antibody (ab115155); Lane 2-Immunoprecipitation with an anti-Cdk8 antibody that recognizes an upstream epitope; Lane 3- Immunoprecipitation with IgG control. An anti-Cdk8 antibody used in lane 2 was used at 0.4 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.

    Anti-Cdk8 antibody (ab115155)参考文献

    ab115155 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab115155.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"