概述

  • 产品名称Anti-Cdk8抗体
    参阅全部 Cdk8 一抗
  • 描述
    兔多克隆抗体to Cdk8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Dog, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cdk8.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; HepG2; Caco2; A549; MCF7; NIH3T3.

性能

应用

Our Abpromise guarantee covers the use of ab106320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cdk8 antibody
    • CDK8 protein kinase antibody
    • CDK8_HUMAN antibody
    • Cell Division Kinase 8 antibody
    • Cell division protein kinase 8 antibody
    • Cyclin Dependent kinase 8 antibody
    • Cyclin-dependent kinase 8 antibody
    • K35 antibody
    • Mediator complex subunit cdk8 antibody
    • Mediator of RNA polymerase II transcription subunit cdk8 antibody
    • Protein kinase K35 antibody
    see all

Anti-Cdk8 antibody 图像

  • All lanes : Anti-Cdk8 antibody (ab106320) at 1 µg/ml (Milk Blocked - 5%)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

Anti-Cdk8 antibody (ab106320)参考文献

ab106320 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab106320.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"