重组
RabMAb

Anti-Cdk7抗体[EP4324] (ab124712)

概述

  • 产品名称
    Anti-Cdk7抗体[EP4324]
    参阅全部 Cdk7 一抗
  • 描述
    兔单克隆抗体[EP4324] to Cdk7
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to residues in Human Cdk7.

  • 阳性对照
    • MCF7, A431, and HeLa cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 功能
      Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
    • 组织特异性
      Ubiquitous.
    • 序列相似性
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
      Phosphorylation of Thr-170 is required for activity.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 39 kDa protein kinase antibody
      • CAK antibody
      • CAK1 antibody
      • Cdk activating kinase antibody
      • CDK-activating kinase 1 antibody
      • CDK-activating kinase antibody
      • cdk7 antibody
      • CDK7_HUMAN antibody
      • CDKN7 antibody
      • Cell division protein kinase 7 antibody
      • Cyclin dependent kinase 7 antibody
      • cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
      • Cyclin-dependent kinase 7 antibody
      • HCAK antibody
      • Homolog of Xenopus MO15 Cdk activating kinase antibody
      • Kinase subunit of CAK antibody
      • MO15 antibody
      • MO15, Xenopus, homolog of antibody
      • P39 Mo15 antibody
      • p39MO15 antibody
      • Serine threonine kinase Stk1 antibody
      • Serine/threonine protein kinase 1 antibody
      • Serine/threonine protein kinase MO15 antibody
      • STK1 antibody
      • TFIIH basal transcription factor complex kinase subunit antibody
      see all

    图片

    • All lanes : Anti-Cdk7 antibody [EP4324] (ab124712) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1 - 2 : Goat anti-Rabbit HRP at 1/2000 dilution


      Predicted band size : 39 kDa

    实验方案

    文献

    ab124712 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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