概述

  • 产品名称Anti-Cdk7抗体
    参阅全部 Cdk7 一抗
  • 描述
    兔多克隆抗体to Cdk7
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 330-346 of Human Cdk7.

  • 阳性对照
    • NIH3T3 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 41.5 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
    Phosphorylation of Thr-170 is required for activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 39 kDa protein kinase antibody
    • CAK antibody
    • CAK1 antibody
    • Cdk activating kinase antibody
    • CDK-activating kinase 1 antibody
    • CDK-activating kinase antibody
    • cdk7 antibody
    • CDK7_HUMAN antibody
    • CDKN7 antibody
    • Cell division protein kinase 7 antibody
    • Cyclin dependent kinase 7 antibody
    • cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
    • Cyclin-dependent kinase 7 antibody
    • HCAK antibody
    • Homolog of Xenopus MO15 Cdk activating kinase antibody
    • Kinase subunit of CAK antibody
    • MO15 antibody
    • MO15, Xenopus, homolog of antibody
    • P39 Mo15 antibody
    • p39MO15 antibody
    • Serine threonine kinase Stk1 antibody
    • Serine/threonine protein kinase 1 antibody
    • Serine/threonine protein kinase MO15 antibody
    • STK1 antibody
    • TFIIH basal transcription factor complex kinase subunit antibody
    see all

Anti-Cdk7 antibody 图像



  • Predicted band size : 41.5 kDa
    ab894 at 1/1000 dilution staining approximately 36kDa band of mouse cdk7 in NIH3T3 whole cell lysate by Western blot (ECL). ab894 at 1/1000 dilution staining approximately 36kDa band of mouse cdk7 in NIH3T3 whole cell lysate by Western blot (ECL).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Cdk7 with ab894 at 4µg/ml. Staining in epithelial cells of intestinal villus.

  • Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-CDK7 antibody (ab894)

Anti-Cdk7 antibody (ab894)参考文献

ab894 has not yet been referenced specifically in any publications.

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