Anti-Cdk6 (phospho Y24)抗体(ab131469)
Key features and details
- Rabbit polyclonal to Cdk6 (phospho Y24)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cdk6 (phospho Y24)抗体
参阅全部 Cdk6 一抗 -
描述
兔多克隆抗体to Cdk6 (phospho Y24) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human colon carcinoma tissue; extracts from 293 cells; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131469 was purified by affinity-chromatography using epitope-specific phospho peptide. The antibody against non-phospho peptide was removed by chromatography using non-phospho peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131469于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 1021 Human
- Entrez Gene: 114483 Rat
- Omim: 603368 Human
- SwissProt: Q00534 Human
- Unigene: 119882 Human
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别名
- CDK 6 antibody
- CDK6 antibody
- CDK6_HUMAN antibody
see all
图片
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All lanes : Anti-Cdk6 (phospho Y24) antibody (ab131469) at 1/500 dilution
Lane 1 : Extract from 293 cells; untreated
Lane 2 : Extract from 293 cells; treated with starvation
Predicted band size: 37 kDa -
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling Cdk6 (phospho Y24) with ab131469 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling Cdk6 (phospho Y24) with ab131469 at 1/50 dilution. The image on the right is preincubated with blocking peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab131469 被引用在 8 文献中.
- Lv T et al. Knockdown of lncRNA DLEU1 inhibits the tumorigenesis of oral squamous cell carcinoma via regulation of miR-149-5p/CDK6 axis. Mol Med Rep 23:N/A (2021). PubMed: 33880596
- Che H et al. p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle. Elife 9:N/A (2020). PubMed: 32125276
- Baptista Moreno Martin AC et al. [10]-Gingerol improves doxorubicin anticancer activity and decreases its side effects in triple negative breast cancer models. Cell Oncol (Dordr) 43:915-929 (2020). PubMed: 32761561
- Zhu J et al. Targeting the polycomb repressive complex-2 related proteins with novel combinational strategies for nasopharyngeal carcinoma. Am J Cancer Res 10:3267-3284 (2020). PubMed: 33163269
- Cao A & Li X Bilobalide protects H9c2 cell from oxygen-glucose-deprivation-caused damage through upregulation of miR-27a. Artif Cells Nanomed Biotechnol 47:2980-2988 (2019). PubMed: 31322008
- Liu C et al. Upregulation of RUNX1 Suppresses Proliferation and Migration through Repressing VEGFA Expression in Hepatocellular Carcinoma. Pathol Oncol Res N/A:N/A (2019). PubMed: 31289995
- Wang M et al. Long non-coding RNA OIP5-AS1 promotes proliferation of lung cancer cells and leads to poor prognosis by targeting miR-378a-3p. Thorac Cancer 9:939-949 (2018). PubMed: 29897167
- Adam MG et al. SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle 14:3734-47 (2015). WB . PubMed: 26654769