概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to Cdk6
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: KDGLCRADQQ YECVAEIGEG AYGKVFKARD LKNGGRFVAL KRVRVQTGEE GMPLSTIREV AVLRHLETFE HPNVVRLFDV CTVSRTDRET KLTLVFE, corresponding to amino acids 3-100 of Human Cdk6

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab54576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.

靶标

  • 功能
    Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDK 6 antibody
    • CDK6 antibody
    • CDK6_HUMAN antibody
    • Cell division protein kinase 6 antibody
    • Crk 2 antibody
    • Crk2 antibody
    • Cyclin dependent kinase 6 antibody
    • Cyclin-dependent kinase 6 antibody
    • MCPH12 antibody
    • MGC59692 antibody
    • p40 antibody
    • PLSTIRE antibody
    • Serine/threonine protein kinase PLSTIRE antibody
    • Serine/threonine-protein kinase PLSTIRE antibody
    • STQTL11 antibody
    see all

图片



  • Predicted band size : 37 kDa

    Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 4 and 6: CDK6 knockout HAP1 cell lysate (20 µg)
    Lanes 1 and 2: Green signal from target – ab54576 observed at 37 kDa
    Lanes 3 and 4: Red signal from loading control – ab8227 observed at 42 kDa
    Lanes 5 and 6: Merged (red and green) signal

    ab54576 was shown to react with CDK6 when CDK6 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK6 knockout samples were subjected to SDS-PAGE. ab54576 and ab8227 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD)  preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 37 kDa
    Cdk6 antibody (ab54576) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

实验方案

文献

This product has been referenced in:
  • Laurenti E  et al. CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16:302-13 (2015). IF . Read more (PubMed: 25704240) »
  • von Witzleben A  et al. Preclinical Characterization of Novel Chordoma Cell Systems and Their Targeting by Pharmocological Inhibitors of the CDK4/6 Cell-Cycle Pathway. Cancer Res 75:3823-31 (2015). Read more (PubMed: 26183925) »

See all 3 Publications for this product

客户评价及客户问答

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Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. As such the concentration is set by these sources. All stock currently held by us does have a concentration of 0...

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