概述

  • 产品名称Anti-CDK5RAP2抗体
    参阅全部 CDK5RAP2 一抗
  • 描述
    兔多克隆抗体to CDK5RAP2
  • 经测试应用适用于: ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1843 and the C-terminus (residue 1893) of Human CDK5RAP2 (NP_060719.4)

  • 阳性对照
    • HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab86340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
  • 应用说明ICC/IF: 1/100 - 1/500. Fix with formaldehyde of methanol. Do not fix with acetone. Permeabilization with Triton-X 100 recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends.
    • 组织特异性Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • 疾病相关Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3) [MIM:604804]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
    • 翻译后修饰Phosphorylated in vitro by CDK5.
    • 细胞定位Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasm. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C48 antibody
      • CDK5 activator-binding protein C48 antibody
      • CDK5 regulatory subunit associated protein 2 antibody
      • CDK5 regulatory subunit-associated protein 2 antibody
      • CDK5RAP2 antibody
      • centrosomal protein 215 kDa antibody
      • Centrosome-associated protein 215 antibody
      • CEP215 antibody
      • CK5P2_HUMAN antibody
      • MCPH3 antibody
      see all

    Anti-CDK5RAP2 antibody 图像

    • ab86340 at 1/250 dilution staining CDK5RAP2 in NBF-fixed asynchronous HeLa cells by Imunofluorescence.
      Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1/100.
    • ab86340 at 1/250 dilution staining CDK5RAP2 in NBF-fixed asynchronous HeLa cells by Imunofluorescence.
      Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1/100.

    Anti-CDK5RAP2 antibody (ab86340)参考文献

    This product has been referenced in:
    • Astuti D  et al. Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility. Nat Genet 44:277-84 (2012). Read more (PubMed: 22306653) »

    See 1 Publication for this product

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