概述

  • 产品名称Anti-CDK5RAP2抗体
    参阅全部 CDK5RAP2 一抗
  • 描述
    兔多克隆抗体to CDK5RAP2
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, African Green Monkey
  • 免疫原

    Synthetic peptide correponding to a region between residues 900 and 950 of human CDK5RAP2

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab70213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 3 - 5µg/mg of lysate.
    WB: 1/2500 - 1/15000. Detects a band of approximately 238 kDa (predicted molecular weight: 215 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends.
    • 组织特异性Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • 疾病相关Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3) [MIM:604804]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
    • 翻译后修饰Phosphorylated in vitro by CDK5.
    • 细胞定位Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasm. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C48 antibody
      • CDK5 activator-binding protein C48 antibody
      • CDK5 regulatory subunit associated protein 2 antibody
      • CDK5 regulatory subunit-associated protein 2 antibody
      • CDK5RAP2 antibody
      • centrosomal protein 215 kDa antibody
      • Centrosome-associated protein 215 antibody
      • CEP215 antibody
      • CK5P2_HUMAN antibody
      • MCPH3 antibody
      see all

    Anti-CDK5RAP2 antibody 图像

    • All lanes : Anti-CDK5RAP2 antibody (ab70213) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from NIH3T3 cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 215 kDa
      Observed band size : 238 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 330 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • Detection of Human CDK5RAP2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 1/4 of IP loaded) using affinity purified rabbit anti-CDK5RAP2 antibody ab70213 at 3 µg/mg of lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.

    Anti-CDK5RAP2 antibody (ab70213)参考文献

    ab70213 has not yet been referenced specifically in any publications.

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