为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Synthetic peptide within Human Cdk5 aa 250-350 (C terminal). The exact sequence is proprietary.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab40773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of frozen PFA-fixed chicken midbrain tissue cyrosection, labelling Cdk5 with ab40773 at a dilution of 1/200 incubated for 16 hours at 4°C in 0.1% PBS Triton X and 3% BSA. Blocking was with 3% BSA incubated for 1 hour at 21°C. Secondary was a goat anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/1000.
Paraffin-embedded human brain tissue stained for Cdk5 with ab40773 at 1/100 dilution.
Flow cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Cdk5 with ab40773 (pink). Negative control Rabbit IgG (green).
ab40773 at 1/50 immunoprecipitating Cdk5 in HeLa whole cell lysate.
For western blotting, the primary antibody was used at a dilution of 1/1000 and a HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"