概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to Cdk5
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length human Cdk5 protein (NP_004926.1)

  • 阳性对照
    • Rat brain tissue lysate. Cell lysate from transfected 293T cells. HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位
    Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cdk 5 antibody
    • Cdk5 antibody
    • CDK5_HUMAN antibody
    • Cell division protein kinase 5 antibody
    • Crk6 antibody
    • Cyclin dependent kinase 5 antibody
    • Cyclin-dependent kinase 5 antibody
    • Protein kinase CDK5 splicing antibody
    • PSSALRE antibody
    • Serine threonine protein kinase PSSALRE antibody
    • Serine/threonine-protein kinase PSSALRE antibody
    • Tau protein kinase II catalytic subunit antibody
    • TPKII catalytic subunit antibody
    see all

图片

  • Anti-Cdk5 antibody (ab88837) at 1 µg/ml + rat brain tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • All lanes : Anti-Cdk5 antibody (ab88837) at 1 µg/ml

    Lane 1 : cell lysate from Cdk5 transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • ab88837 at 10 µg/ml staining Cdk5 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.

文献

ab88837 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (293 cells)
Specification
293 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

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提交于 Jul 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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