Cdk4 overexpression 293T裂解液(全细胞) (ab94290)


  • 产品名称
    Cdk4 overexpression 293T裂解液(全细胞)
  • 描述
    Cdk4 overexpression裂解液
  • 常规说明
    ab94290 is a 293T cell transfected lysate in which Human Cdk4 has been transiently over-expressed using a pCMV-Cdk4 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用
    适用于: WBmore details


  • 背景
    Function: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Disease: Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain. PTM: Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.


Our Abpromise guarantee covers the use of ab94290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Cdk4 overexpression 293T lysate (whole cell) 图像

  • ab94290 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Cdk4 antibody (ab54515) at 1/500 dilution

    Lane 1 : Cdk4 overexpression 293T lysate (whole cell) (ab94290)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Cdk4 overexpression 293T lysate (whole cell) (ab94290)参考文献

ab94290 has not yet been referenced specifically in any publications.

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