概述

  • 产品名称
    Anti-Cdk4抗体[AF10A4]
    参阅全部 Cdk4 一抗
  • 描述
    小鼠单克隆抗体[AF10A4] to Cdk4
  • 经测试应用
    适用于: ChIP, Sandwich ELISA, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length Cdk4 protein (Human) purified from E.coli.

  • 阳性对照
    • Ramos, SK-N-MC, MCF-7, Jurkat, PC12 and NIH3T3 cell lysates
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR51720 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab75511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

For sandwich ELISA, use this antibody as Capture at 1/200 dilution with Rabbit polyclonal to Cdk4 (ab7955) as Detection.

WB 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • 疾病相关
    Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
  • 细胞定位
    Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cdk 4 antibody
    • cdk4 antibody
    • CDK4 protein antibody
    • CDK4_HUMAN antibody
    • Cell division kinase 4 antibody
    • Cell division protein kinase 4 antibody
    • CMM 3 antibody
    • CMM3 antibody
    • Crk3 antibody
    • Cyclin dependent kinase 4 antibody
    • Cyclin-dependent kinase 4 antibody
    • Melanoma cutaneous malignant 3 antibody
    • MGC14458 antibody
    • p34 cdk4 antibody
    • PSK J3 antibody
    • PSK-J3 antibody
    see all

图片

  • Standard Curve for Cdk4 (Analyte: Cdk4 protein (T7 Tag) (ab40586)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AF10A4] to Cdk4 (ab75511) at 1/200 dilution. and Detector Antibody Rabbit polyclonal to Cdk4 (ab7955) at 0.5µg/ml.
  • All lanes : Anti-Cdk4 antibody [AF10A4] (ab75511) at 1/2000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : SK-N-MC cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH3T3 cell lysate


    Predicted band size : 34 kDa
    Observed band size : 34 kDa

文献

This product has been referenced in:
  • Chen Z  et al. Suppressive effect of formononetin on platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells. Exp Ther Med 12:1901-1907 (2016). Human . Read more (PubMed: 27588108) »
  • Hunsucker SA  et al. Peptide/MHC tetramer-based sorting of CD8? T cells to a leukemia antigen yields clonotypes drawn nonspecifically from an underlying restricted repertoire. Cancer Immunol Res 3:228-35 (2015). Read more (PubMed: 25576336) »

See all 2 Publications for this product

客户评价及客户问答

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Unfortunately, the concentration of the anti-Cdk4 antibody [AF10A4] ab75511 has not been determined. This is because this antibody ...

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