RabMAb

Anti-Cdk2 (phospho Y15)抗体[EPR2233Y] (ab76146)

概述

  • 产品名称Anti-Cdk2 (phospho Y15)抗体[EPR2233Y]
    参阅全部 Cdk2 一抗
  • 描述
    兔单克隆抗体[EPR2233Y] to Cdk2 (phospho Y15)
  • 特异性ab76146 detects Cdk2 phosphorylated on tyrosine 15.
  • 经测试应用适用于: IHC-P, WB, IPmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cdk2 (phospho Y15).

  • 阳性对照
    • WB: HeLa cell and mouse spleen tissue lysates. IHC-P: Human transitional cell carcinoma of bladder and rat colon tissues. IP: HeLa cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

     

    Alternative versions available:

    Anti-Cdk2 antibody (Alexa Fluor® 488) [EPR2233Y] (ab190193)
    Anti-Cdk2 antibody (Alexa Fluor® 647) [EPR2233Y] (ab190562)

性能

应用

Our Abpromise guarantee covers the use of ab76146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

WB 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/10000.

IP 1/100.

For unpurified use at 1/50.

  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] 图像

    • All lanes : Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146) at 1/50000 dilution (purified)

      Lane 1 : Untreated HeLa cell lysate
      Lane 2 : HeLa cell lysate - treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 34 kDa


      Exposure time : 10 seconds

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of Cdk2 (pY15) peptide (Lane 1) and Cdk2 non-phospho peptide (Lane 2) labelling Cdk2 (pY15) with purified ab76146 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue labelling Cdk2 (phospho Y15) with purified ab76146 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. A prediluted HRP polymer-conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Cdk2 (phospho Y15) with purified ab76146 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. A prediluted HRP polymer-conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • ab76146 (purified) at 1/100 immunoprecipitating Cdk2 (phospho Y15) in HeLa cell lysates (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146)参考文献

    This product has been referenced in:
    • Liang J  et al. PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. Nat Commun 7:12431 (2016). WB ; Human . Read more (PubMed: 27485204) »
    • Lin PC  et al. Identification of Phosphorylated Cyclin-Dependent Kinase 1 Associated with Colorectal Cancer Survival Using Label-Free Quantitative Analyses. PLoS One 11:e0158844 (2016). Read more (PubMed: 27383761) »

    See all 7 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (10 %)
    Sample Human Cell lysate - whole cell (Hela cells)
    Specification Hela cells
    Treatment 2 ´M MK-1775
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Remi Buisson

    Verified customer

    提交于 Oct 27 2014

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (osteosarcoma cells)
    Specification osteosarcoma cells
    Treatment lane 1 control, lane 2 siRNA for CDK2, 48h
    Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    提交于 Sep 26 2014

    Thank you for your inquiry. I have confirmed with the laboratory that we have not tested cross-reactivity with phospho-CDK1. Sequence analysis shows that the immunogen of ab76146 shares 100% identity with human CDK1. Thus, I would predict cross-reac...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"