Anti-Cdk2 (phospho T14)抗体[EP2234Y] (ab68265)


  • 产品名称
    Anti-Cdk2 (phospho T14)抗体[EP2234Y]
    参阅全部 Cdk2 一抗
  • 描述
    兔单克隆抗体[EP2234Y] to Cdk2 (phospho T14)
  • 宿主
  • 特异性
    This antibody detects Cdk2 phosphorylated on Threonine 14.
  • 经测试应用
    适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Rat, Human, Chinese hamster
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.

  • 阳性对照
    • HeLa cell lysate, human tonsil tissue. IHC/P: Rat esophagus (FFPE)
  • 常规说明

    A trial size is available to purchase for this antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents




Our Abpromise guarantee covers the use of ab68265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP 1/30.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
    • 序列相似性
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all


    • IHC image of ab68265 staining Cdk2 in rat esophagus formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68265, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/20000 dilution

      Lane 1 : HeLa cell lysate, untreated.
      Lane 2 : HeLa cell lysate, treated with hydroxyurea.

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 34 kDa
      Observed band size: ~34 kDa (why is the actual band size different from the predicted?)

    • Human tonsil tissue stained with ab68265 at 1/100-1/250 dilution.


    This product has been referenced in:
    • Li J  et al. Phosphorylation of MCM3 Protein by Cyclin E/Cyclin-dependent Kinase 2 (Cdk2) Regulates Its Function in Cell Cycle. J Biol Chem 286:39776-85 (2011). WB ; Human . Read more (PubMed: 21965652) »
    • Abdullah C  et al. Expression analysis and molecular targeting of cyclin-dependent kinases in advanced melanoma. Cell Cycle 10:977-88 (2011). WB ; Human . Read more (PubMed: 21358262) »

    See all 3 Publications for this product


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