概述

  • 产品名称
    Anti-Cdk2抗体[AN4.3]
    参阅全部 Cdk2 一抗
  • 描述
    小鼠单克隆抗体[AN4.3] to Cdk2
  • 特异性
    Reacts with human, mouse and xenopus cdk2. Does not recognise other cdks.
  • 经测试应用
    适用于: WB, ELISA, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
  • 免疫原

    Human cdk2 (cyclin-dependent kinase 2) recombinant protein

  • 表位
    The epitope is at the C-terminus.
  • 阳性对照
    • HeLa nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab6433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt 1/160. Incubate for 1 hour.

  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
    • 序列相似性
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all

    图片



    • Predicted band size : 34 kDa

      This image is courtesy of Julian Gannon, CRUK.

      Western blot using ab6433 at 5µg/ml.

      Lane 1: HeLa

      Lane 2: 293

      Lane 3: mouse myeloid cells.

      Western blot using ab6433 at 5µg/ml.

      Lane 1: HeLa

      Lane 2: 293

      Lane 3: mouse myeloid cells.

    • ab6433 staining human MDA-MB-436 breast cancer cells by Flow Cytometery. Cells were treated with Trypsin/EDTA for 5 minutes, sample buffer prepared as 5%FCS in PBS and gating was done on live non apoptotic cells. The primary antibody diluted 1/160 and incubated with sample for 1 hour at 4°C. A FITC conjugated horse polyclonal to mouse IgG, diluted 1/200 was used as secondary.

      See Abreview

    文献

    This product has been referenced in:
    • Zhang X  et al. MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/ß-catenin pathway. Am J Cancer Res 6:2631-2640 (2016). WB ; Human . Read more (PubMed: 27904776) »
    • Li VC  et al. Gap 1 phase length and mouse embryonic stem cell self-renewal. Proc Natl Acad Sci U S A 109:12550-5 (2012). Read more (PubMed: 22802651) »

    See all 5 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (MDA-MB-436 breast cancer cells)
    Specification
    MDA-MB-436 breast cancer cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsin/EDTA for 5 minutes
    Sample buffer: 5% FCS in PBS
    Permeabilization
    No
    Gating Strategy
    live (non apoptotic cells) were selected
    Username

    Dr. Penelope Ottewell

    Verified customer

    提交于 Dec 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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