概述

  • 产品名称Anti-Cdk2抗体
    参阅全部 Cdk2 一抗
  • 描述
    兔多克隆抗体to Cdk2
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from an internal region of Human Cdk2.

  • 阳性对照
    • Extracts from HUVEC, HepG2 or Jurkat cells; HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab70012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

靶标

Anti-Cdk2 antibody 图像

  • All lanes : Anti-Cdk2 antibody (ab70012) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from Jurkat cells
    Lane 4 : Extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells using 1/500 ab70012. Right: the sample was pre-incubated with the immunizing peptide

Anti-Cdk2 antibody (ab70012)参考文献

ab70012 has not yet been referenced specifically in any publications.

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