概述

  • 产品名称Anti-Cdk2抗体
    参阅全部 Cdk2 一抗
  • 描述
    兔多克隆抗体to Cdk2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Dog, Chinese Hamster, Syrian Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Cdk2.

    (Peptide available as ab71917.)

  • 阳性对照
    • This antibody gave a positive signal in the following Lysates: HeLa Whole Cell, Rat Thymus Tissue, HeLa Whole Cell - Bleomycin Treated (20U/ml), Jurkat Whole Cell, Y79 Whole Cell

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab64669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

靶标

Anti-Cdk2 antibody 图像



  • Predicted band size : 35 kDa

    Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)

    Lanes 2, 4 and 6: CDK2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Green signal from target – ab64669 observed at 34 kDa

    Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa

    Lanes 5 and 6: Merged (red and green) signal

    ab64669 was shown to react with CDK2 when CDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK2 knockout samples were subjected to SDS-PAGE. ab64669 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Thymus (Rat) Tissue Lysate
    Lane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

Anti-Cdk2 antibody (ab64669)参考文献

ab64669 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab64669.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"