概述

  • 产品名称Anti-CDK11 / CDC2L2抗体
    参阅全部 CDK11 / CDC2L2 一抗
  • 描述
    兔多克隆抗体to CDK11 / CDC2L2
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    This information is considered to be commercially sensitive.

  • 阳性对照
    • HeLa whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibody was affinity purified using an epitope specific to CDK11 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 90 kDa).
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Appears to play multiple roles in cell cycle progression, cytokinesis and apoptosis. The p110 isoforms have been suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. The p58 isoform may act as a negative regulator of normal cell cycle progression.
  • 组织特异性Expressed ubiquitously. Some evidence of isoform-specific tissue distribution.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰During apoptosis, induced by Fas or tumor necrosis factor, specific CKD11 p110 isoforms are cleaved by caspases to produce a protein (p110C) that contains the C-terminal kinase domain of the CDK11 proteins.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD11A_HUMAN antibody
    • CDC2L2 antibody
    • CDC2L3 antibody
    • CDK11 antibody
    • CDK11 p110 antibody
    • CDK11 p46 antibody
    • CDK11 p58 antibody
    • CDK11A antibody
    • Cell division cycle 2 like 2 (PITSLRE proteins) antibody
    • Cell division cycle 2-like protein kinase 2 antibody
    • Cell division protein kinase 11A antibody
    • Cyclin-dependent kinase 11A antibody
    • Galactosyltransferase-associated protein kinase p58/GTA antibody
    • p58GTA antibody
    • PITSLRE antibody
    • PITSLRE B antibody
    • PITSLRE protein kinase beta antibody
    • PITSLRE serine/threonine-protein kinase CDC2L2 antibody
    • PITSLREB antibody
    see all

Anti-CDK11 / CDC2L2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling CDK11 / CDC2L2 with ab19393 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 90 kDa

    Whole cell lysate (20 and 50 µg for WB; 10 mg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK11 antibody (ab19393) used at 0.05 µg/ml for WB (A) and at 1 µg/mg lysate for IP (B). Detection: A) Chemiluminescence with an exposure time of 3 minutes. B) Immunoprecipitate was resolved on 4 to 20% gel, which was stained with Coomassie, and the band that is CDK11 was verified by mass spectrometry.

    Whole cell lysate (20 and 50 µg for WB; 10 mg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK11 antibody (ab19393) used at 0.05 µg/ml for WB (A) and at 1 µg/mg lysate for IP (B). Detection: A) Chemiluminescence with an exposure time of 3 minutes. B) Immunoprecipitate was resolved on 4 to 20% gel, which was stained with Coomassie, and the band that is CDK11 was verified by mass spectrometry.
  • Immunofluorescence analysis of HeLa cells, staining CDK11 / CDC2L2 with ab19393.

    Cells were fixed with formaldehyde, permeabilized with 0.5% NP-40 and blocked with 10% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/100 in 10% horse serum in PBS) for 1 hour at 20°C. An AlexaFluor®555-conjugated donkey anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

Anti-CDK11 / CDC2L2 antibody (ab19393)参考文献

This product has been referenced in:
  • Eldridge A  et al. Manganese superoxide dismutase interacts with a large scale of cellular and mitochondrial proteins in low-dose radiation-induced adaptive radioprotection. Free Radic Biol Med 53:1838-47 (2012). WB . Read more (PubMed: 23000060) »
  • Wilkinson S  et al. The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy. Autophagy 7:1295-301 (2011). WB . Read more (PubMed: 21808150) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Alistair Davies

Verified customer

提交于 May 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 100 µg
Specification Liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Liver)
Specification Liver
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.5 % NP40
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jul 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"