概述

  • 产品名称
    Anti-CDK1 (phospho Y15)抗体
    参阅全部 CDK1 一抗
  • 描述
    兔多克隆抗体to CDK1 (phospho Y15)
  • 特异性
    This antibody is specific for CDK1.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human CDK1 aa 5-54.
    Database link: P06493

  • 阳性对照
    • HepG2 cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab47594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ELISA 1/10000.

靶标

  • 功能
    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • 组织特异性
    Isoform 2 is found in breast cancer tissues.
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式
    CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • 别名
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Anti-CDK1 (phospho Y15) antibody 图像

  • Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + COS7 cells

    Predicted band size : 34 kDa
  • Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/1000 dilution

    Lane 1 : untreated HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with nocodazole (500ng/mL) for 16 h

    Lysates/proteins at 20 µg per lane.

    Secondary
    polyclonal goat HRP conjugated at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 34 kDa
    Observed band size : 34 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous abreview.

    Blocking: 5% milk for 1 hour at 25°C.

    See Abreview

实验方案

Anti-CDK1 (phospho Y15) antibody (ab47594)参考文献

This product has been referenced in:
  • Chang L  et al. Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy. BMC Cancer 16:190 (2016). WB ; Human . Read more (PubMed: 26951044) »
  • Li HB  et al. Mitochondrial Ribosomal Protein L10 Associates with Cyclin B1/Cdk1 Activity and Mitochondrial Function. DNA Cell Biol 35:680-690 (2016). WB ; Human . Read more (PubMed: 27726420) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Treatment
1 - untreated, 2- nocodazole 500 ng/ml 16 h
Gel Running Conditions
Reduced Denaturing (8% Tris Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 13 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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